5KLQ
Crystal structure of HopZ1a in complex with IP6 and CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.131, 82.295, 86.268 |
| Unit cell angles | 64.20, 76.69, 89.35 |
Refinement procedure
| Resolution | 47.566 - 3.400 |
| R-factor | 0.2392 |
| Rwork | 0.234 |
| R-free | 0.28150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HopZ1a |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.570 |
| High resolution limit [Å] | 3.400 |
| Number of reflections | 15997 |
| <I/σ(I)> | 4.8 |
| Completeness [%] | 97.3 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20% PEG10000. 100mM HEPES, pH 7.0, 200 mM ammonium acetate |
