5KLA

Crystal structure of the drosophila Pumilio RNA-binding domain in complex with hunchback RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	194.904, 29.537, 62.034
Unit cell angles	90.00, 101.25, 90.00

Refinement procedure

Resolution	34.458 - 1.140
R-factor	0.1615
Rwork	0.160
R-free	0.17400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h3d
RMSD bond length	0.007
RMSD bond angle	0.950
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.160
High resolution limit [Å]	1.140	3.090	1.140
Rmerge	0.045	0.036	0.387
Rmeas	0.050
Rpim	0.021
Total number of observations	531939
Number of reflections	127177
<I/σ(I)>	11.8
Completeness [%]	99.6	99.8	97.4
Redundancy	4.2	6.3	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15% (w/v) PEG 3350, 0.1 M bis-tris, pH 6.5 and 0.2 M NH4OAc