5KL7

Wilms Tumor Protein (WT1) ZnF2-4Q369R in complex with carboxylated DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	67.458, 77.797, 35.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.231 - 1.579
R-factor	0.1652
Rwork	0.164
R-free	0.19360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r2r
RMSD bond length	0.011
RMSD bond angle	1.357
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2400: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.650
High resolution limit [Å]	1.579	1.579
Rmerge	0.089	0.464
Number of reflections	26124
<I/σ(I)>	17.7	2.81
Completeness [%]	99.9	99.6
Redundancy	6.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	20% (w/v) polyethylene glycol 3350, 40 mM citric acid, and 60 mM bistris-propane pH 6.5