5KL6
Wilms Tumor Protein (WT1) Q369R ZnF2-4 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-26 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.982, 66.008, 35.567 |
| Unit cell angles | 90.00, 90.52, 90.00 |
Refinement procedure
| Resolution | 29.031 - 1.641 |
| R-factor | 0.1748 |
| Rwork | 0.173 |
| R-free | 0.20640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r2r |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.306 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2257: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.075 | 0.439 |
| Number of reflections | 19712 | |
| <I/σ(I)> | 20 | 2.1 |
| Completeness [%] | 91.6 | 50.4 |
| Redundancy | 6 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 20% (w/v) PEG 3350, 0.2 M Na-malonate, pH 7.0 |
