5KL5
Wilms Tumor Protein (WT1) ZnF2-4 Q369H in complex with carboxylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.493, 77.211, 35.794 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.263 - 2.289 |
| R-factor | 0.1957 |
| Rwork | 0.193 |
| R-free | 0.24630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r2r |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.659 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2400: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.370 |
| High resolution limit [Å] | 2.289 | 2.289 |
| Rmerge | 0.113 | 0.480 |
| Number of reflections | 8861 | |
| <I/σ(I)> | 19.9 | 2.76 |
| Completeness [%] | 99.5 | 95.6 |
| Redundancy | 10.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 20% (w/v) polyethylene glycol 3350, 0.2 M ammonium acetate, and 0.1 M bistris-HCl, pH 6.5 |
