5KL3
Wilms Tumor Protein (WT1) ZnF2-4 Q369H in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-09 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.223, 66.069, 35.646 |
| Unit cell angles | 90.00, 91.75, 90.00 |
Refinement procedure
| Resolution | 29.223 - 1.449 |
| R-factor | 0.1634 |
| Rwork | 0.162 |
| R-free | 0.18230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r2r |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.133 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2257: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.449 | 1.449 |
| Rmerge | 0.082 | 0.610 |
| Number of reflections | 27228 | |
| <I/σ(I)> | 16.3 | 1.9 |
| Completeness [%] | 88.0 | 43.3 |
| Redundancy | 4.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 12% (w/v) PEG 3350, 4% (v/v) Tacsimate, pH 8.0 |
