5KL2
Wilms Tumor Protein (WT1) ZnF2-4 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-09 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.107, 65.089, 35.702 |
| Unit cell angles | 90.00, 93.42, 90.00 |
Refinement procedure
| Resolution | 29.115 - 1.692 |
| R-factor | 0.1656 |
| Rwork | 0.164 |
| R-free | 0.19330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r2r |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.194 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2439: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.106 | 0.424 |
| Number of reflections | 17475 | |
| <I/σ(I)> | 16.3 | 2.9 |
| Completeness [%] | 97.8 | 84.2 |
| Redundancy | 6.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 289 | 16% (w/v) PEG 3350, 20 mM Citric acid, 80 mM Bis-tris propane, pH 8.8 |
