5KKJ
2.0-Angstrom In situ Mylar structure of hen egg-white lysozyme (HEWL) at 293 K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2016-03-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.064, 79.064, 38.336 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.953 - 2.001 |
R-factor | 0.1536 |
Rwork | 0.149 |
R-free | 0.19960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zix |
RMSD bond length | 0.007 |
RMSD bond angle | 0.995 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.953 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.047 | 0.132 |
Number of reflections | 8173 | |
<I/σ(I)> | 11.28 | 7.53 |
Completeness [%] | 95.0 | 67 |
Redundancy | 1.9 | 1.7 |
CC(1/2) | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 4.5 | 293 | 100 mM NaOAc 1 M NaCl, 30% (v/v) PEG 400 |