5KKH
2.1-Angstrom In situ Mylar structure of bacteriorhodopsin from Haloquadratum walsbyi (HwBR) at 100 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.110, 61.270, 119.030 |
| Unit cell angles | 90.00, 116.26, 90.00 |
Refinement procedure
| Resolution | 29.446 - 2.125 |
| R-factor | 0.2138 |
| Rwork | 0.213 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ei4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.774 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.450 | 2.200 |
| High resolution limit [Å] | 2.125 | 2.130 |
| Rmerge | 0.107 | 0.250 |
| Number of reflections | 35323 | |
| <I/σ(I)> | 6.26 | 3.66 |
| Completeness [%] | 91.0 | 70 |
| Redundancy | 1.8 | 1.8 |
| CC(1/2) | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | 293 | 8% (v/v) Tacsimate, 20% (v/v) PEG 3350 |
