5KKB

Structure of mouse Golgi alpha-1,2-mannosidase IA and Man9GlcNAc2-PA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	94.955, 131.487, 87.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.840 - 1.774
R-factor	0.1611
Rwork	0.161
R-free	0.19140
RMSD bond length	0.009
RMSD bond angle	0.876
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	41.760
High resolution limit [Å]	1.774
Number of reflections	101615
<I/σ(I)>	3.22
Completeness [%]	95.0
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	283	100 mM MES, 50 mM ammonium sulfate, 15% PEG 4000, pH 6.5