5KK5
AsCpf1(E993A)-crRNA-DNA ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 195.737, 195.737, 125.206 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 105.473 - 3.289 |
| R-factor | 0.2182 |
| Rwork | 0.216 |
| R-free | 0.25480 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.071 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.473 | 3.430 |
| High resolution limit [Å] | 3.289 | 3.290 |
| Rmerge | 0.101 | 1.530 |
| Number of reflections | 37141 | |
| <I/σ(I)> | 14.1 | 1.36 |
| Completeness [%] | 99.5 | 96.6 |
| Redundancy | 5.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris, pH 7.0, 30% PEG600, 0.5 M (NH4)2SO4 |
