5KK0Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-E |
| Synchrotron site | APS |
| Beamline | 21-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 118.760, 124.961, 203.911 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.610 - 2.800 |
| R-factor | 0.22699 |
| Rwork | 0.225 |
| R-free | 0.26371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ou9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.397 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.610 |
| High resolution limit [Å] | 2.790 |
| Number of reflections | 75385 |
| <I/σ(I)> | 7.1 |
| Completeness [%] | 99.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | Sodium Chloride, BisTris Propane, Sodium Polyacrylate 2100, Triton X-100 |
