5KJS

Crystal Structure of Arabidopsis thaliana HCT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979482
Spacegroup name	P 32 2 1
Unit cell lengths	99.910, 99.910, 83.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.222 - 2.203
R-factor	0.1829
Rwork	0.180
R-free	0.24080
RMSD bond length	0.009
RMSD bond angle	1.273
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	86.620
High resolution limit [Å]	1.970
Rmerge	0.089
Number of reflections	34427
<I/σ(I)>	16.6
Completeness [%]	99.9
Redundancy	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.24	277.15	0.5 M ammonium acetate, 0.1M MOPSO-NaOH, pH 7.24, 18% PEG 8000