5KJB
Synechocystis apocarotenoid oxygenase (ACO) mutant - Glu150Asp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-E |
| Synchrotron site | APS |
| Beamline | 21-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 118.420, 124.950, 203.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.580 - 2.810 |
| R-factor | 0.20319 |
| Rwork | 0.202 |
| R-free | 0.23334 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ou9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.331 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.580 |
| High resolution limit [Å] | 2.810 |
| Number of reflections | 74574 |
| <I/σ(I)> | 7.9 |
| Completeness [%] | 99.9 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | BisTris Propane, sodium polyacrylate 2100, sodium chloride |
