5KJA
Synechocystis apocarotenoid oxygenase (ACO) mutant - Trp149Ala
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 118.140, 125.260, 203.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.620 - 2.800 |
| R-factor | 0.19998 |
| Rwork | 0.198 |
| R-free | 0.23905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ou9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.375 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.620 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.190 | 0.204 |
| Number of reflections | 75013 | |
| <I/σ(I)> | 11 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.2 | 3.77 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | BisTris Propane, sodium polyacrylate 2100, sodium chloride |
