5KJ4

Crystal Structure of Mouse Protocadherin-15 EC9-10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 31
Unit cell lengths	143.541, 143.541, 95.599
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.980 - 3.350
R-factor	0.15943
Rwork	0.158
R-free	0.19590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xhz
RMSD bond length	0.010
RMSD bond angle	1.078
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.410
High resolution limit [Å]	3.350	3.350
Rmerge	0.256	0.931
Number of reflections	31590
<I/σ(I)>	6.96	2.03
Completeness [%]	100.0	100
Redundancy	5.9	5.8
CC(1/2)	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277.15	0.095M HEPES (pH7.5), 0.19M CaCl2, 26.6% v/v PEG 400