5KIS
YenB/RHS2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-02-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54179 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.607, 132.765, 155.361 |
| Unit cell angles | 90.00, 103.85, 90.00 |
Refinement procedure
| Resolution | 32.510 - 2.400 |
| R-factor | 0.2183 |
| Rwork | 0.216 |
| R-free | 0.25990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4igl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.305 |
| Data reduction software | XDS (Oct 15, 2015) |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.510 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.305 | 0.443 |
| Number of reflections | 113222 | |
| <I/σ(I)> | 9.4 | 0.6 |
| Completeness [%] | 99.2 | 98.2 |
| Redundancy | 10.1 | 10 |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 22% PEG 6000, 0.2 M TAPS pH 8.5 |
