5KI8
PSEUDO T4 LYSOZYME MUTANT - Y88PHE-BR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2013-04-01 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.013, 60.013, 95.945 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.700 - 1.550 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.991 |
| Data reduction software | d*TREK (9.9.9.4L) |
| Data scaling software | d*TREK (9.9.9.4L) |
| Phasing software | PHASER |
| Refinement software | PHENIX (DEV_1951) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.700 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.026 | 0.310 |
| Number of reflections | 28167 | |
| <I/σ(I)> | 16.1 | 6.3 |
| Completeness [%] | 95.0 | 71 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291.15 | 2.4M KPO4, 50MM 2-HYDROXYETHYLDISULFIDE, 50MM 2-MERCAPTOETHANOL, PH 6.9 |
