5KI3
PSEUDO T4 LYSOZYME MUTANT - Y18PHE-BR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2014-07-13 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.589, 59.589, 95.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.400 - 1.650 |
| R-factor | 0.21 |
| Rwork | 0.200 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.967 |
| Data reduction software | d*TREK (9.9.9.6L) |
| Data scaling software | d*TREK (9.9.9.6L) |
| Phasing software | PHASER |
| Refinement software | PHENIX (DEV_1839) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.400 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.210 | 0.759 |
| Number of reflections | 23668 | |
| <I/σ(I)> | 4.6 | 1 |
| Completeness [%] | 98.0 | 92 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 291.15 | 2.0M KPO4, 50MM 2-HYDROXYETHYLDISULFIDE, 50MM 2-MERCAPTOETHANOL, PH 6.7 |
