5KI1

PSEUDO T4 LYSOZYME MUTANT - Y18F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-17
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	59.763, 59.763, 95.119
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.880 - 1.460
R-factor	0.182
Rwork	0.180
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.006
RMSD bond angle	1.010
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (DEV_1839)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.880
High resolution limit [Å]	1.460
Rmerge	0.065
Number of reflections	34257
<I/σ(I)>	31
Completeness [%]	98.0
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	291.15	2.4M KPO4, 50MM 2-HYDROXYETHYLDISULFIDE, 50MM 2-MERCAPTOETHANOL, PH 6.7