5KFB

Human DNA polymerase eta-DNA ternary complex: reaction with 1 mM Mn2+ for 90s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.380, 98.380, 82.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.719 - 1.550
R-factor	0.1767
Rwork	0.174
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		1.590
High resolution limit [Å]	1.550	6.930	1.550
Rmerge	0.050	0.023	0.452
Number of reflections	64154
<I/σ(I)>	20.59	64.2	3.54
Completeness [%]	98.0	88.1	99.5
Redundancy	4.8
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	298	17% PEG 2000MME, 0.1 M MES