5KEV
Vibrio parahaemolyticus VtrA/VtrC complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97935, 0.97927, 0.97943 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 211.012, 211.012, 211.012 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.609 - 2.700 |
| R-factor | 0.2649 |
| Rwork | 0.260 |
| R-free | 0.29810 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.422 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.609 | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 7.190 | 2.650 |
| Rmerge | 0.076 | 0.032 | 1.724 |
| Number of reflections | 12322 | ||
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 99.9 | 98.8 | 100 |
| Redundancy | 36.1 | 29.2 | 36.5 |
| CC(1/2) | 0.934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 1.0 M lithium sulfate, 0.5 M ammonium sulfate, 0.1 M sodium citrate 28.7% ethylene glycol |
