5KEV

Vibrio parahaemolyticus VtrA/VtrC complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97935, 0.97927, 0.97943
Spacegroup name	F 4 3 2
Unit cell lengths	211.012, 211.012, 211.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.609 - 2.700
R-factor	0.2649
Rwork	0.260
R-free	0.29810
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.422
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.609	50.000	2.700
High resolution limit [Å]	2.650	7.190	2.650
Rmerge	0.076	0.032	1.724
Number of reflections	12322
<I/σ(I)>	10.4
Completeness [%]	99.9	98.8	100
Redundancy	36.1	29.2	36.5
CC(1/2)	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	1.0 M lithium sulfate, 0.5 M ammonium sulfate, 0.1 M sodium citrate 28.7% ethylene glycol