5KER

Deer mouse recombinant hemoglobin from high altitude species

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97870
Spacegroup name	P 1 21 1
Unit cell lengths	53.182, 229.618, 53.277
Unit cell angles	90.00, 119.12, 90.00

Refinement procedure

Resolution	29.560 - 2.202
R-factor	0.1935
Rwork	0.193
R-free	0.20170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4h2l
RMSD bond length	0.006
RMSD bond angle	0.852
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((1.10.1_2155))
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.560
High resolution limit [Å]	2.200
Rmerge	0.089
Number of reflections	65478
<I/σ(I)>	9.3
Completeness [%]	71.0
Redundancy	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		299	26% (w/v) PEG 3350 in 50 mM sodium/potassium phosphate, pH 8.0