5KEK
Structure Determination of a Self-Assembling DNA Crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 67.926, 67.926, 59.262 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.467 - 3.098 |
| R-factor | 0.2107 |
| Rwork | 0.204 |
| R-free | 0.25990 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.316 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.065 | 0.342 |
| Number of reflections | 3030 | |
| <I/σ(I)> | 74.9 | 3 |
| Completeness [%] | 99.4 | 93.5 |
| Redundancy | 9.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 50 mM cacodylate pH 6.0, 20 mM MgCl2, 1.0 mM spermine, and 15% ethanol |
