5KEF
Structure of hypothetical Staphylococcus protein SA0856 with zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 91.867, 92.611, 95.259 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.130 - 2.230 |
| R-factor | 0.2028 |
| Rwork | 0.201 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data scaling software | autoPROC |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.130 | 2.238 |
| High resolution limit [Å] | 2.230 | 2.231 |
| Rmerge | 0.065 | 0.950 |
| Number of reflections | 20145 | |
| <I/σ(I)> | 17.6 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15% PEG 3350, Mg acetate, 3% ethanol, pH 7.7 |
