5KEC
Structure of K. pneumonia MrkH in its apo state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.800, 93.450, 206.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.565 - 1.949 |
| R-factor | 0.2055 |
| Rwork | 0.203 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ked |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.799 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.571 | 2.070 |
| High resolution limit [Å] | 1.949 | 1.949 |
| Number of reflections | 29115 | |
| <I/σ(I)> | 7.2 | 1.5 |
| Completeness [%] | 90.4 | 79.6 |
| Redundancy | 2.7 | 2.1 |
| CC(1/2) | 0.995 | 0.647 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 30% PEG 600, 0.1 M Hepes pH 7.5, 50 mM lithium sulphate, 10% w/v glycerol |
