5KCB
The structure of SAV2435 bound to ethidium bromide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-09 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 73.720, 73.720, 68.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.462 - 2.101 |
R-factor | 0.1999 |
Rwork | 0.199 |
R-free | 0.21940 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.909 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 32.462 |
High resolution limit [Å] | 2.101 |
Number of reflections | 11441 |
<I/σ(I)> | 38.04 |
Completeness [%] | 99.5 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2.4 M ammonium sulfate, 0.1 M Tris pH 8.5 |