5KCA

Crystal structure of the Cbln1 C1q domain trimer in complex with the amino-terminal domain (ATD) of iGluR Delta-2 (GluD2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96862
Spacegroup name	P 61 2 2
Unit cell lengths	142.680, 142.680, 276.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.023 - 3.100
R-factor	0.2057
Rwork	0.204
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kc5 5kc8
RMSD bond length	0.005
RMSD bond angle	0.966
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_1772)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.050	3.180
High resolution limit [Å]	3.100	3.100
Rmerge	0.224	1.377
Number of reflections	30725
<I/σ(I)>	9.5	1.5
Completeness [%]	99.6	99.8
Redundancy	7.6	7.5
CC(1/2)	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	60% (v/v) tacsimate pH 7.0 (1.8305 M malonic acid, 0.25 M ammonium citrate tribasic, 0.12 M succinic acid, 0.3 M DL-malic acid, 0.4 M sodium acetate trihydrate, 0.5 M sodium formate, and 0.16 M ammonium tartrate dibasic)