5KC5
Crystal structure of the Cbln1 C1q domain trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.07300 |
| Spacegroup name | P 6 |
| Unit cell lengths | 83.420, 83.420, 50.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.550 - 2.351 |
| R-factor | 0.1814 |
| Rwork | 0.177 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gr3 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.559 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1772) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.570 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.126 | 1.263 |
| Number of reflections | 8497 | |
| <I/σ(I)> | 15.2 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 8.1 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium malonate pH 7.0, 20% (w/v) polyethylene glycol 3350 |
