5KBP
The crystal structure of an alpha-mannosidase from Enterococcus faecalis V583
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 127.834, 127.834, 223.716 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.1889 |
| Rwork | 0.187 |
| R-free | 0.23150 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.465 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_2386) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.077 | 0.704 |
| Number of reflections | 83104 | |
| <I/σ(I)> | 26.1 | 2.4 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2 Li2SO4, 0.1M Tris:HCl, 2.0M (NH4)2SO4 |
