5KAQ
Crystal structure of broadly neutralizing Influenza A antibody 31.a.83 in complex with Hemagglutinin Hong Kong 1968.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 279.435, 154.283, 157.937 |
| Unit cell angles | 90.00, 116.86, 90.00 |
Refinement procedure
| Resolution | 41.880 - 3.514 |
| R-factor | 0.2721 |
| Rwork | 0.270 |
| R-free | 0.31260 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.532 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.500 |
| Number of reflections | 58736 |
| <I/σ(I)> | 13 |
| Completeness [%] | 79.0 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | .1M Tris, pH 8.5, 5.0% (w/v) PEG-8000, 20% (v/v) PEG-300, and 10% (v/v) glycerol |
