5K9V
Protein Tyrosine Phosphatase 1B (1-301), open state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-03 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 88.526, 88.526, 72.992 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.333 - 1.898 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.20590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sug |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.010 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.898 | 5.160 | 1.900 |
| Rmerge | 0.064 | 0.040 | 0.369 |
| Total number of observations | 498785 | ||
| Number of reflections | 26228 | ||
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 98.9 | 99.9 | 89.8 |
| Redundancy | 19 | 20.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277 | 0.1 M Tris, pH 7.8, 0.2 M MgCl2, 18% PEG8000 |
