5K9T

SecA-N68, a C-terminal truncation of the SecA ATPase from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.105535
Spacegroup name	P 1 21 1
Unit cell lengths	67.065, 64.450, 87.967
Unit cell angles	90.00, 105.85, 90.00

Refinement procedure

Resolution	17.916 - 2.600
R-factor	0.2009
Rwork	0.196
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3BXZ and 5K94
RMSD bond length	0.006
RMSD bond angle	0.769
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	84.623	40.868	2.730
High resolution limit [Å]	2.590	8.200	2.590
Rmerge		0.028	0.511
Number of reflections	22456
<I/σ(I)>	8.8	20	1.5
Completeness [%]	99.4	97.8	96.8
Redundancy	4	3.9	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris-HCl pH 8.0, 18% PEG 3000, and 0.9% w/v cadaverine; 18 mg/mL protein concentration. Cryoprotectant included 18% PEG 3000 and 20% PEG 200