5K8Y
Structure of the Mus musclus Langerin carbohydrate recognition domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 143.310, 143.310, 143.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.320 - 2.400 |
| R-factor | 0.19792 |
| Rwork | 0.196 |
| R-free | 0.23384 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kqg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.237 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.780 | |
| Number of reflections | 19343 | |
| <I/σ(I)> | 4.8 | 2.1 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 20 | 19.5 |
| CC(1/2) | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.1 M MES, pH 6.0, 30% (v/v) polyethylene glycol 600, 5% (w/v) polyethylene glycol 1000, and 10% (v/v) glycerol |
