5K8R

Structure of human clustered protocadherin gamma B3 EC1-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	128.881, 161.229, 53.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.350 - 2.500
R-factor	0.2106
Rwork	0.205
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zi9
RMSD bond length	0.006
RMSD bond angle	0.618
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_2276)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.350	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.133	1.962
Number of reflections	19844
<I/σ(I)>	10.06	0.98
Completeness [%]	92.0	42
Redundancy	6.8	6.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M HEPES pH 7 4% ethylene glycol 5% PEG500MME