5K7W
Crystal structure of the catalytic domain of Mettl3/Mettl14 complex with SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97934 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 101.942, 101.942, 118.009 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.793 - 1.650 |
R-factor | 0.1608 |
Rwork | 0.160 |
R-free | 0.17940 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.924 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.650 |
Number of reflections | 75134 |
<I/σ(I)> | 32.8 |
Completeness [%] | 100.0 |
Redundancy | 16.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1 M Tris pH 8.0, 20% PEG3350 |