5K7D

The structure of native pistol ribozyme, bound to Iridium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.1052
Spacegroup name	P 32 2 1
Unit cell lengths	56.573, 56.573, 88.297
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.000 - 2.680
Rwork	0.190
R-free	0.24000
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.300	2.810
High resolution limit [Å]	2.680	2.680
Rpim	0.038	0.618
Number of reflections	8819
<I/σ(I)>	14.9	1.2
Completeness [%]	99.8
Redundancy	6.2	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Na-cacodylate, pH 6.5, Mg(OAc)2 0.2 M and 32% MPD and supplemented with 5 mM Ir(NH3)63+Cl3