5K7A
Crystal structure of laccase fron Thermus thermophilus HB27 (sodium nitrate 1.5 min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9791 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 93.744, 110.332, 96.484 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.719 - 1.500 |
R-factor | 0.1688 |
Rwork | 0.168 |
R-free | 0.19090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xu9 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.906 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.719 | 1.600 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.077 | 0.466 |
Number of reflections | 79274 | |
<I/σ(I)> | 15.76 | 3.49 |
Completeness [%] | 97.3 | 86.7 |
Redundancy | 4.5 | 4.39 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1 M HEPES, pH 7.5, 70% MPD |