5K71
apo Dbr1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.110, 142.163, 214.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 107.150 - 2.570 |
| R-factor | 0.1967 |
| Rwork | 0.194 |
| R-free | 0.24220 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4pef |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.640 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.150 | 2.630 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.110 | 0.920 |
| Number of reflections | 134191 | |
| <I/σ(I)> | 12.6 | 1.6 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 3350, lithium sulfate, glycerol, bis-tris |
