5K6V
Sidekick-1 immunoglobulin domains 1-4, crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0052 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 158.189, 158.189, 53.525 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.740 - 3.208 |
| R-factor | 0.2088 |
| Rwork | 0.207 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Sdk1 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.489 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.290 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.147 | 0.520 |
| Number of reflections | 11639 | |
| <I/σ(I)> | 8.2 | 1.5 |
| Completeness [%] | 90.7 | 72.9 |
| Redundancy | 4.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 295 | 10% PEG8000, 0.01M zinc chloride, 0.1M HEPES, pH 7.5 |
