5K6S

The structure of the PP2A B56 subunit BubR1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	53.231, 107.811, 117.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.522 - 2.794
R-factor	0.2013
Rwork	0.198
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jak
RMSD bond length	0.002
RMSD bond angle	0.686
Data reduction software	XDS
Data scaling software	Aimless (0.5.17)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.520	2.940
High resolution limit [Å]	2.790	2.790
Rmerge	0.072	1.382
Number of reflections	17235
<I/σ(I)>	16.8
Completeness [%]	98.4	93.2
Redundancy	6.6	6.7
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.75	298	8% PEG 6K, 0.8 M HEPES, 0.8 M LiCl