5K63
Crystal structure of N-terminal amidase C187S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97960 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 134.355, 134.355, 119.792 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.276 - 2.500 |
R-factor | 0.1909 |
Rwork | 0.185 |
R-free | 0.24680 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.866 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.276 |
High resolution limit [Å] | 2.500 |
Number of reflections | 19185 |
<I/σ(I)> | 56.3 |
Completeness [%] | 99.9 |
Redundancy | 15.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M Mg acetate tetrahydrate, 0.2M Na cacodylate pH6.5, 20% (w/v) PEG 8000 |