Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 102.700, 305.600, 84.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.400 - 2.369 |
R-factor | 0.2099 |
Rwork | 0.207 |
R-free | 0.26260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.040 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 76.400 |
High resolution limit [Å] | 2.369 |
Number of reflections | 45617 |
<I/σ(I)> | 17 |
Completeness [%] | 83.9 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 20% PEG 8000, 20% PEG 400, 100 mM Magnesium chloride, 100 mM Tris pH 8.5 |