5K58
Structure of the K. pneumonia SlmA-DNA complex bound to the C-terminal of the cell division protein FtsZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 84.800, 84.800, 161.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.435 - 2.772 |
| R-factor | 0.2453 |
| Rwork | 0.247 |
| R-free | 0.26750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.923 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.435 | |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.127 | 0.403 |
| Number of reflections | 32384 | |
| <I/σ(I)> | 7 | 1.9 |
| Completeness [%] | 98.2 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 29% PEG 3000, 0.1 M Tris HCl pH 7.0, 0.2 M NaCl |
