5K4T
Three-dimensional structure of L-threonine 3-dehydrogenase from Trypanosoma brucei refined to 2.1 angstroms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-08-04 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9173 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 91.759, 91.759, 93.598 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.070 - 2.100 |
R-factor | 0.1637 |
Rwork | 0.161 |
R-free | 0.21630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 2.004 |
Data scaling software | xia2 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.073 |
High resolution limit [Å] | 2.100 |
Rmerge | 0.054 |
Number of reflections | 22705 |
<I/σ(I)> | 33.6 |
Completeness [%] | 99.9 |
Redundancy | 12.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M MES, 0.2M ammonium sulphate, 30% PEG w/v 5000 MME |