5K38
Crystal structure of Retinoic acid receptor-related orphan receptor (ROR) gamma ligand binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 61 |
| Unit cell lengths | 99.346, 99.346, 129.411 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.020 - 2.050 |
| R-factor | 0.18581 |
| Rwork | 0.185 |
| R-free | 0.20693 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l0l |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.706 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 86.040 |
| High resolution limit [Å] | 2.050 |
| Rmerge | 0.077 |
| Number of reflections | 41810 |
| <I/σ(I)> | 16.31 |
| Completeness [%] | 97.0 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.4M Potassium sodium tartrate tetrahydrate |
