5K2Q

Lysozyme with nano particles

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	77.355, 77.355, 37.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.339 - 1.100
R-factor	0.1541
Rwork	0.153
R-free	0.18030
RMSD bond length	0.018
RMSD bond angle	1.853
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.120
High resolution limit [Å]	1.100	2.980	1.100
Rmerge	0.079	0.050	0.816
Rmeas	0.083	0.053	0.855
Rpim	0.024	0.016	0.250
Total number of observations	513159
Number of reflections	46637
<I/σ(I)>	9.2
Completeness [%]	99.1	99.9	89.1
Redundancy	11	11.2	9.8
CC(1/2)		0.998	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1M Tris-HCl pH8.5, 1.3M Ammonium Tartrate Dibasic