5K2N

lysozyme with nano particles

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	77.520, 77.520, 37.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.380 - 1.400
R-factor	0.1393
Rwork	0.137
R-free	0.17870
RMSD bond length	0.018
RMSD bond angle	1.684
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.420
High resolution limit [Å]	1.400	3.790	1.400
Rmerge	0.065	0.030	0.468
Rmeas	0.068	0.031	0.491
Rpim	0.020	0.009	0.146
Total number of observations	260426
Number of reflections	23263
<I/σ(I)>	9.6
Completeness [%]	99.9	100	99.9
Redundancy	11.2	10.9	11.1
CC(1/2)		1.000	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1M Tris-HCl pH8.5, 1.4M Ammonium Tartrate Dibasic