5K2K

Crystal structure of lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	77.361, 77.361, 37.517
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.340 - 1.650
R-factor	0.181
Rwork	0.178
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.018
RMSD bond angle	1.760
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.096	0.545
Rmeas	0.100	0.071
Rpim	0.030	0.022
Total number of observations	163851
Number of reflections	14184
<I/σ(I)>	8.6
Completeness [%]	100.0	100
Redundancy	11.6	11.7
CC(1/2)		0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1M Tris:HCl pH8.5, 1.4M Ammonium Tartrate Dibasic