5K21
Pyocyanin demethylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03318 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.330, 72.980, 79.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.980 - 1.800 |
Rwork | 0.168 |
R-free | 0.19510 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.980 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.176 |
Number of reflections | 35992 |
<I/σ(I)> | 13.29 |
Completeness [%] | 100.0 |
Redundancy | 25.9 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% w/v PEG 8000, 200 mM NaCl, 200 micromolar 1-hydroxyphenazine, 1% DMSO, and 100 mM sodium phosphate dibasic/citric acid, pH 4.2 mixed 1:1 with 5 mg/mL protein in 20 mM Tris, pH 7.6 |